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Produits

MP Premium Dextran Sulfate de Sodium (DSS) Recherche sur les maladies inflammatoires chroniques intestinales

Sulfate de dextran , Sodium dextran sulfate

1 clic Devis

FastPrep® Kits d’extraction et de purification Kits d’extraction et de purification DNA/RNA et protéines

ARN , Protéines

1 clic Devis

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Actualités

Directe Nouvelle réponse - Le 20/12/2021

Quelle est la référence pour les billes du broyeur Fastprep?

MP BIOMEDICALS

a répondu :

Nous disposons de 16 types de billes différentes permettant de s'adapter au type d'échantillon à broyer. Vous trouverez plus d'information sur notre page web: https://www.mpbio.com/eu/life-sciences/sample-preparation-0/fastprep-lysing-matrices

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Nouvelle réponse - Le 05/01/2021

Quelles sont les différents moyens de déterminer les cellules viables dans une population cellulaire?

MP BIOMEDICALS

a répondu :

The FastCounting cell counting kit from MP Biomedicals provides a sensitive and convenient colorimetric method to determine cell viability in cell proliferation and cytotoxicity assays.

FastCounting-Kit-Manual

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Nouvelle réponse - Le 14/02/2019

Je cherche de billes de broyage en verre et zirconium/silice pour application biologie moléculaire. Des recommandations?

MP BIOMEDICALS

a répondu :

Bonjour,

MP Biomedicals fournit un vaste choix de billes de broyage en verre et zirconium/silice pour vos applications biologie moléculaire.

N'hésitez pas à nous contacter notre spécialiste au 03.88.67.54.45

L'équipe MP BIOMEDICALS

www.mpbio.com

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193 entreprises participent à l'évènement FORUM LABO PARIS 2019

Le 12/02/2019

FORUM LABO PARIS 2019

26 MARS

Voir le planning des exposants

MP BIOMEDICALS

a partagé une offre d'emploi

Le 08/02/2019

Nous recrutons un.e commercial.e terrais pour la Région Rhône-Alpes

Annonce Commercial(e) Terrain Région Rhône Alpes

MP BIOMEDICALS

Contrat : CDI

Lieux : Rhône Alpes

Le 07/02/2019 Postuler

Sciences et techniques

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MP Biomedicals commercialise plus de 55 000 produits et instruments pour la recherche.

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74%

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Documents

FastCounting-Kit-Manual

www.mpbio.com For colorimetric quantitation of viable cell number in proliferation and cytotoxicity assays FastCounting TM Cell Counting Kit Instruction Manual Storage: Store at 2-8 °C (1 year) or -20 °C (long-term) with protection from light. Cat. No. 092690131, 092690132 2 Product Description FastCounting TM Cell Counting Kit (1mL reagent for 100 tests, and 5 mL for 500 tests) provides a sensitive and convenient method to determine cell viability in cell proliferation and cytotoxicity assays. The kit uses a water-soluble tetrazolium salt, WST-8 (i.e. 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) to quantify the number of live cells by producing a yellow colored formazan dye upon bio reduction by dehydrogenase activities in cells. The amount of formazan dye generated by the bio reduction inside cells is directly proportional to the number of living cells. The detection sensitivity of FastCounting Cell Kits is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1. Due to its stability and extremely low cytotoxicity, FastCounting is suitable for longer incubation periods (such as 24 or 48 hours). This product is for R&D use only. FastCounting TM Cell Counting Kit 3 Key Benefits Highest sensitivity dye for cell viability Lowest cytotoxicity among tetrazolium reagents Simple procedures without thawing reagents Long shelf life and easy storage Kit Contents 1 x 1 mL reagent for 100 tests (092690131) 1 x 5 mL reagent for 500 tests (092690132) Storage FastCounting Cell Kit is stable for over 1 year at 2-8 °C with protection from light. It can also be stored at -20 °C long-term. Avoid repeated thawing and freezing, which can cause an increase in background. FastCounting TM Cell Counting Kit 4 General protocol for cell number determination (Figure 1): 1. Inoculate cell suspensions (100 µL/well) in a well plate under the required cell culture conditions. Add 10 µL of FastCounting solution to each well of the plate. Avoid introducing any bubbles into the wells, which may interfere with O.D. readings. 2. Incubate the plate for 1-4 hours in the incubator. 3. Measure the absorbance at 450 nm using a microplate reader. Cell Proliferation and Cytotoxicity Assay 1. Dispense 100 µL of cell suspension (5,000 cells/well) in a well plate. 2. Add 10 µL of various concentrations of substances to be tested to the plate. 3. Incubate the plate for an appropriate length of time (e.g. 6, 12, 24 or 48 hours) in an incubator. 4. Add 10 µL of FastCounting solution to each well of the plate (avoid generating bubbles). 5. Incubate the plate for 1-4 hours in an incubator. 6. Measure the absorbance at 450 nm using a microplate reader. 1. Add FastCounting solution 2. Incubate for 1-4 hours3. Measure O.D. at 450 nm FastCounting TM Cell Counting Kit MP Biomedicals Americas: 800.854.0530 | custserv@mpbio.com Europe: 00800.7777.9999 | custserv.eur@mpbio.com Japan: 03.6667.0730 | sales.japan@mpbio.com Singapore: 65.6775.0008 | enquiry_ap@mpbio.com South Korea: 82.2.425.5991 | info.korea@mpbio.com Australia: 61.2.8824.2100 | aus.cs@mpbio.com China: 86.4000.150.0680 | mpchina@mpbio.com India: 91.22.27636921/22/24 | info.india@mpbio.com New Zealand: 64.9.912.2460 | nzsales@mpbio.com LEARN MORE www.mpbio.com

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SIREN / SIRET	331 914 721 000 13
Chiffre d'affaires	5 à 10 M€
Effectif	201 à 500
APE / NAF	7219Z
Capital	50 à 200 K€
N° TVA	FR 84 331 914 721